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KEYENCE bz-x 810 fluorescence microscope
Bz X 810 Fluorescence Microscope, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bz-x+810+fluorescence+microscope/fluorescence+microscope+bz+9000/pm40422892-96-19-23
Average 90 stars, based on 1 article reviews
bz-x 810 fluorescence microscope - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Fluorescence:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Microscopy:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Staining:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Software:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Knock-In:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Transgenic Assay:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Generated:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Quantitative RT-PCR:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Recombinant:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Reporter Assay:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Luciferase:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Control:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Plasmid Preparation:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

RNA Sequencing:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Flow Cytometry:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).

Imaging:

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously.59

Article Title: Establishment of primary and immortalized fibroblasts reveals resistance to cytotoxic agents and loss of necroptosis-inducing ability in long-lived Damaraland mole-rats.
Article Snippet: Images were acquired with a BZ-X 810 fluorescence microscope (Keyence) and positive cells were counted with a BZ-X Image Analyzer (Keyence).

Article Title: Reduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, Japan).

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion
Article Snippet: Images were collected using Keyence BZ-X 810 fluorescence microscope.

Article Title: Effects of Maillard Reaction Products on Skeletal Muscle Cells: An In Vitro Study Using C2C12 Myotubes.
Article Snippet: Finally, the samples were mounted using ProLongTM Diamond Antifade Mountant containing DAPI (Thermo Fisher Scientific) and visualized using a BZ-X 810 fluorescence microscope (KEYENCE).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: LysM-Cre Knockin Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004781 CAG-Cre ERT2 Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:004682 Adipoq-Cre Transgenic mice The Jackson Laboratory RRID:IMSR_JAX:010803 Mafbf/gfp Generated In-House https://doi.org/10.1128/ MCB.00001–06 Oligonucleotides Primers for RT-qPCR (See Table S3) This Paper N/A Cre8, 50-CCCAGAAATGCCAGATTACG-30 This Paper N/A NLSCre: 50-CCCAAGAAGAAGAGGGTGTCC-30 This Paper N/A LysM-forward 50-CCATTATTTCACAGCAGCATTGC-30 This Paper N/A LysM-rev 50-GCTGACTCCATAGTAGCCAG-30 This Paper N/A Recombinant DNA pGL4.10[luc2] Vector Promega, Madison, Wisconsin, Cat# E665A Dual-Luciferase Reporter Assay System Promega, Madison, Wisconsin, Cat# E1910 pRL Renilla Luciferase Control Reporter Vectors Promega, Madison, Wisconsin, Cat# E2231 MAFB overexpressing vector Generated In-House https://doi.org/10.1128/ MCB.00001-06 Software and algorithms STAR version = STAR_2.6.1a_08-27 https://github.com/ N/A R-Programming Language (For RNA seq Analysis) https://www.r-project.org/ N/A Enrichr tool https://maayanlab.cloud/Enrichr/ N/A Partek Flow software version 10.0.22.1111 https://www.partek.com/partek-flow/ N/A BZ-X810 analyzer software Keyence Corporation, Osaka, Japan BZ-X810 ImageJ http://imagej.net N/A CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Qiagen Ingeunity Pathway Analysis QIAGEN, Redwood City, California Version 107193442 Cytoflex flow cytometer Beckman coulter, Brea, California V2-B5-R3 CytExpert software 2.4 Beckman coulter, Brea, California 2.4 Other Rodent Rectal thermistor skSATO, Tokyo, Japan Cat# SK-1260 IR camera compact pro Seek thermal, Inc. Santa Barbara, USA N/A Mlx90614ESF IR-Sensor Melexis, Ypres, Belgium Cat# Mlx90614ESF Indirect calorimeter for mice Muromachi Kikai Co., Ltd., Tokyo, Japan Cat# MK-5000RQ Keyence BZ-X 810 fluorescence microscope Keyence Corporation, Osaka, Japan BZ-X 810 Ultra microscope II Miltenyi Biotec, Bergisch Gladbach, Germany N/A iBright Imaging Systems Thermofisher Scientific, Waltham, Massachusetts N/A

Article Title: Reduced lung metastasis in endothelial cell-specific TGF-β type II receptor-deficient mice with decreased CD44 expression
Article Snippet: After the nuclei as needed were stained with 2 μg/mL DAPI (Dojindo 728 Laboratories, Kumamoto, Japan) for 5 min, the samples were washed three times with PBS, and 729 the fluorescence signals were visualized by BZ-X 810 fluorescence microscope (Keyence, Osaka, 730 Japan).

Article Title: MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue.
Article Snippet: Images were acquired using a Keyence BZ-X 810 fluorescence microscope, analyzed, and quantified using BZ-X810 analyzer software as described previously or ImageJ (Fiji).59 The list of primary and secondary antibodies is provided in (Key resource table).



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